Posts Tagged "Biological"

Biodetection Technologies 5th Edition – Technological Response to Biological Threats —- Aarkstore Enterprise

With an emphasis on rapid detection and low-cost identification, this publication provides solutions to the challenges presented by the evolving pattern of complex biological threat agents of various origins.

Completely updated with the latest information, including narratives, charts, graphs and question & answers, this essential reference tool addresses the following important topics:

– PCR and Non-PCR Based Detection
– Reagent and Reagentless Based Detection
– Point-of-Care Toxin & Pathogen Detection
– Utilizing Bacteriophage for Biological Threat Response
– Real-World Sample Preparation for Toxin & Pathogen Detection

Table of Contents : Chapter 1
The Neccesity for Improving Biodetection Technologies
Robert Hooks, Deputy Assistant Secretary for WMD and Biodefense, U.S. Department of Homeland Security
In the biodefense area, DHS efforts focus on providing leadership by coordinating with other Federal partners, state/local/tribal jurisdictions, the private sector and the international community. In addition to developing technologies for Biowatch or NBIS, which are two DHS program areas, improved technology is equally important for our partners and is critical to improving the nation’s capabilities in the biodefense arena.

Chapter 2
SAFE: Sequencing for Avian Flu Epidemic
Niveen Mulholland, PhD, Senior Scientist, MRI-NCR, Midwest Research Institute*
We have developed a system for detecting mutations that would give rise to potential pandemic-causing influenza strains. The system, SAFE: Sequencing for Avian Flu Epidemic, first uses real time RT-PCR to detect H5, the highly pathogenic avian influenza most likely to cause a pandemic. We next use pyrosequencing to detect codon changes encoding amino acids known to define human versus avian influenza signatures. The SAFE real time RT-PCR assay specifically detects H5 in a multiplex reaction designed to detect a region of the Matrix gene common to all Influenza A subtypes. The H5 primer/probe set is specific; it does not cross react with other Influenza A subtypes tested. The M primer/probe set serves as an internal control, detecting all subtypes tested. Pyrosequencing assays were developed to screen, at the nucleotide level, for 52 amino acids changes defined by Chen et al (2006) to be avian- or human- specific. A library has been built to screen the sequence data generated and properly identify the strain in question as a potential threat. This surveillance system described here will allow the global community to monitor for H5N1 and for mutations that will render the virus more infective and virulent to humans. *In collaboration with: N.Waybright, E.Petrangelo, P.Lowary & J.Bogan

Chapter 3
Next Generation Automated Multi-Target Detection Platform for Closer to Source Diagnostics
Todd Ritter, CEO of Applied Science & Technology, Idaho Technology Inc.
Idaho Technology has developed a highly multiplexed detection system capable of concurrently identifying and genetically discriminating dozens of viruses and bacteria. The syringe-loaded system utilizes a flexible plastic pouch combining automated sample preparation, reverse transcription for RNA viruses, and two-stage nested multiplex PCR performing 120 discreet analyses simultaneously. Capable of processing a variety of sample types, the small, lightweight diagnostic system represents a next generation in automated detection systems.

Chapter 4
Rapid Multiplexed Nucleic Acid and Antibody Based Sensor for Biothreat Detection
Michael R. Meyer, Director of Laboratories, ICx Biosystems
RapidPlex is a fully automated, triggered confirmation system designed for detection and identification of bacteria, virus, and toxin threats in 10 minutes. The RapidPlex system provides simultaneous, multiplexed detection of protein and DNA/RNA markers through parallel antibody and nucleic acid-based assays. Antibody-based detection utilizes multiplexed sandwich assays on spectrally encoded microspheres followed by high resolution imaging of individual beads for detection of spores/cells captured on the bead surfaces as well as detection of toxins and viruses coating the bead surfaces. The nucleic acid-based process includes automated cell lysis and purification, followed by multiplexed DNA/RNA amplification and detection of the DNA amplicons on spectrally encoded microspheres.

Chapter 5
Multiplex Detection of Biothreat Agents by Fluidic Force Discrimination
Gary W. Long, PhD, Vice President and Senior Scientist, Tetracore, Inc.
Fluidic Force discrimination (FFD) has recently been applied to the detection of Biothreat agents. In this talk, we will describe the multiplex detection of 3 or more agents by an immunoassay developed for FFD, and demonstrate that sensitivity of detection is similar or greater than that obtained in lateral flow devices and by ELISA. We will also describe improvements to the sensitivity of detection of nucleic acids using this technology.

Chapter 6
Use of CANARY™ for Rapid, Automated Collection and Analysis of Bioaerosol Samples
Thomas Hazel, PhD, Vice President Research, Innovative Biosensors, Inc.
CANARY™ is a cell-based technology that enables rapid identification of bacterial, viral, and toxin targets in liquid or aerosol samples. In this presentation we describe the testing and validation of our new BioFlash™ instrumentation, designed to provide ‘detect-to-protect’ capability for environmental monitoring and building security applications. This platform enables automated collection, detection, and simultaneous identification of up to 21 target aerosol agents with increased speed and sensitivity.

Chapter 7
SAW RFID Biosensors for Ubiquitous Molecular Recognition
William D. Hunt, PhD, Professor, School of Electrical and Computer Engineering, Georgia Institute of Technology*
Research by Hunt’s group at Georgia Tech has demonstrated that an acoustic wave biosensor with a biolayer coating can detect analytes such as Bascillus spores, cocaine, TNT/RDX in the gaseous phase. A new embodiment of this approach involves a SAW RFID/biosensor, which can passively sense and report on molecules in its environment. The SAW RFID/biosensor system involves a main interrogation unit transmitting an RF signal to a passive SAW structure located a short distance away. The device combined with the antenna re-transmits a reflected acoustic wave, which has been perturbed by the on-chip molecular recognition events. *In collaboration with: P.J.Edmonson, D.D.Stubbs, Zen Sensing LLC; D.Bertieri, Penn United Technologies Inc.

Chapter 8
TIRF-EC Biosensors with Reagentless Bioassays for Rapid and Accurate Detection of Pathogens
Alexander N. Asanov, PhD, President & Scientific Director, TIRF Technologies
This presentation will describe revolutionary new portable and handheld biosensors based on Total Internal Reflection Fluorescence (TIRF) combined with ElectroChemistry (TIRF-EC). TIRF-EC sensors are capable of detecting hundreds of DNA/RNA and protein signatures of multiple pathogens in a matter of several seconds. The detection limit is at the level of single molecules. In conjunction with reagentless assays TIRF-EC sensors require no or minimum sample preparation stages. The sensors are suitable for a variety of biomedical point-of-care and biodefense field applications.

Chapter 9
Cost-Efficient Technological Solutions for Warning-Type Bioaerosol Detection
Victor N. Morozov, PhD, Research Professor, Manager of a Laboratory of Detection & Diagnostics, George Mason University, The National Center for Biodefense and Infectious Diseases (NCBID)
Rapid ultra-sensitive assay of aerosolized pathogens presents a tremendous challenge in biodetection. NCBID has developed two key technologies solving major ‘bottle-necks’ in such detection: (i) energy-consuming collection of aerosol and (ii) slow, diffusion-controlled immunoassay. We demonstrated that collection of aerosol on water-soluble nano-filters combined with electrophoresis-assisted immunoassay and active detection of collected pathogens with magnetic beads enable detection of zeptomolar amounts of pathogens (150-500 molecules or viruses) in 2-3 minutes.

For more information, please visit :

http://www.aarkstore.com/reports/Biodetection-Technologies-5th-Edition-Technological-Response-to-Biological-Threats-1747.html

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Cure for insomnia movie and How to reprogram a biological clock for insomniac so you can sleep again

What\’s the Longest movie ever Made.

(Your life buddy!) is the answer to this question. Someone, a director, by the name of

John Henry Timmis IV, shot a movie back in 1987 reflecting on how life and just natural life can be it\’s own movie. The most lush, plush, green, refreshing movie you will ever experience. In the film poet LD Groban quoted a poem of many many pages. It was interlaced with images of sexual fantasies and exploits of a past. This was the directors choice to show life in this way because he saw that the images in life and the words in life are sometimes all we have.

In The movie LD Groban (poet L.D. Groban) recites his own poem of 4,080 pages. It\’s his life \’s work which is natuarally the only cure for insomnia, chronic insomnia, cataplexy, and narcolepsy

So what\’s the cure for insomnia? How do you reprogram biological clocks for insomniacs so they can sleep again. You know let me first start by saying that Narcolepsy sufferers do this reprograming of there biological clocks naturally. What they don\’t do is reprogram their biological clocks so they can stay up. Okay that seems obvious right?

Let\’s look at the facts about the movie.

The film The Cure For Insomnia mostly consists of poet L.D. Groban reciting his own poem of 4,080 pages.

the Cure For Insomnia is an American movie

It was first played in its entirety at The School Of The Art Institute in Chicago, Illinois from January 31 to Feb. 3, 1987

the movie Cure For Insomnia

was released January 31, 1987.

The Cure for Insomnia is the longest movie ever made at a total running time of 87 hours.

Cure for Insomnia consists of L.D. Groban reciting his own poem, inter linked with X-rated film footage and music videos.ChaCha!

The movie was directed by

John Henry Timmis IV and he wrote and directed the 1987 movie “The Cure for Insomnia”.

It\’s runtime is 5220 minutes. That\’s 87 hours!

Do you know what happens in the brain when you have narcolepsy?

Okay now pay attention, this is deep and if you have doubts at all, You really should be asking a professional other than me. with that said, here are the basics

Narcolepsy means you have no orexins, no fighting genes against sleep.

researchers think this is caused by our auto-immune systems destroying them during the fetal development of that particular part of the brain, possibly in response to exposure to a virus. our brains are missing a particular peptide (amino acids) called hypocretins or orexins. (those born in march have a higher rate of being born with this, partly because the mother is in her second trimester during the big cold/flu season…while those born in later September have the smallest, which puts the pregnant mom in her second trimester during a low cold/flu time of year.) Not in Flu season, So the resistance is low. These orexins regulate functions like the body\’s ability to keep its self up and stay awake.

Vigorous excercise several times a day, Caffeine, ephedrine, pseudoephedrine are the over the counter stimulants for Narcolepsy.

The circus of ever sprouting medication for sleep disorders are only part of the cure. The real cure is your life\’s work. Do and perform your life\’s work. In The movie LD Groban (poet L.D. Groban) recites his own poem of 4,080 pages. His life\’s work should reprogram biological clocks for insomniacs and for that matter narcoleps so you can order your bodies natural sleep cycle again.

Don\’t take medication geared towards mental health issues lightly. You don\’t want to get in to all that if you can help it. Do and perform your life\’s work. Many of these drugs are addictive or they have very serious side effects. This includes

antidepressant Elavil, which is WAY too strong for many. It makes many terribly drowsy and lethargic, not good for someone with narcolepsy!

Tofranil (generic: imipramine) which works well for some because its an antidepressant.

and Ritalin is disliked by all. Tamiflu is prescribed for insomnia.All of these medicines should be subscribed by a doctor. Just say no if you can help, and you can. Do and perform your life\’s work.

Now with insomnia

Your body should adjust to the new wake up time and let you go to sleep when it needs to.

Set your alarm to one time (the time you want to wake up each morning) and follow it strictly.

Ironically the cure for insomnia perhaps is the same cure for narcolepsy.

Do and perform your life\’s work.

-To help you get to sleep/and stay up just enough and to give the body energy to disipline the mind to sleep enough is as follows from all diagnosis:

-make sure there is no noise, no light, smells fine and the room temperature is a cool 65 –degrees and your comfortable in bed.

-exercise in the morning to help you get to sleep/and stay up just enough

-during the day get lots of sunlight

-take a warm bath and have a warm glass of milk or cup of chamomile tea before sleep

don\’t have any caffeine. be sure to drink plenty of water throughout the day.

-don\’t have a big meal before going to sleep but also don\’t go to sleep on an empty stomach. have a small snack, such as yogurt (which also contains tryptophan).

- don\’t watch tv or use the computer for at least a half hour before sleep. Instead do something in a dimmer light to help you get to sleep/and stay up just enough to help you get to sleep/and stay up just enough

By making small lifestyle changes like having a fixed

daily routine, relaxing and eating properly, insomnia can be

cured.

Insomnia (sleeplessness) is due to stress, dietary and medical

problems.

Achieving discipline, order and rest control means you need to go to bed at the same time every night regardless of whether you feel tired or not & once in bed – minimal tv,reading.

Sleep is all about developing a schedule to maintain a normal cycle. Your body needs to establish that the bed is for sleep & nothing else.

No matter what you decide to do, you need to have a professional observing the effects the drug has on your brain and your body. The people answering your question on this site, cannot make these observations even if they know what they are talking about (don\’t take that for granted). TALK TO A PROFESSIONAL!!!

Today prepare to fight. Prepare to fight to do your life\’s work. fight who? fight the urge and the compulsion to self destruct and to stay the same. stop the urge and the inclination to tell yourself that “You can\’t sleep” or “I just fall asleep out of no where”. Fight your own inner creative battles. what do you love to do. what are you passionate about. Wher do you go when the world seems like it just disappears? What is your spot in this world and what is your territory. In The movie LD Groban (poet L.D. Groban) recites his own poem of 4,080 pages. It\’s his life \’s work which is natuarally the only cure for insomnia, chronic insomnia, cataplexy, and narcolepsy

TALK TO A PROFESSIONAL!!! and then do what you can do naturally which is do your life\’s work.

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